Anaerobic Biodegradation of Chloroform under Methanogenic Conditions

by Devesh, Univ of Cincinnati, Cincinnati, United States,
Munish Gupta, Univ of Cincinnati, Cincinnati, United States,
Makram T. Suidan, Univ of Cincinnati, Cincinnati, United States,
Gregory D. Sayles, Univ of Cincinnati, Cincinnati, United States,

Document Type: Proceeding Paper

Part of: Critical Issues in Water and Wastewater Treatment


The degradation of chloroform is studied for two different methanogenic cultures grown on acetate and methanol exclusively, as the primary substrates. In chemostats, chloroform was fed at different concentrations as high as 2000 μg/l along with the primary substrate and chloroform degradation greater than 98% was observed. The kinetics of degradation of chloroform and the primary substrate were investigated using BMP tests and it was seen that the methanol-fed methanogenic culture exhibited higher rates of chloroform degradation than the acetate-fed methanogenic culture. Besides, chloroform inhibited acetate degradation at any concentration while methanol inhibition was observed only for chloroform concentrations higher than 800 μg/l.

Subject Headings: Biological processes | Chemical degradation | Substrates | Acetate | Organic compounds | Anaerobic processes | Water pollution | Kinetics

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