Fluorometer Calibration Curves and Nomographs

by Thomas A. Butts,

Serial Information: Journal of the Sanitary Engineering Division, 1969, Vol. 95, Issue 4, Pg. 705-714

Document Type: Journal Paper


The relationship of rhodamine B dye fluorescence to water temperature is confirmed, and a procedure for calibrating a fluorometer for time-of-travel dye studies is described. Calibration nomographs for the four aperture settings designed into fluorometers are developed from experimentally derived temperature-fluorescence curves. The use of the nomographs expedites and simplifies field work and eliminates the need for standard dye solutions for determining unknown dye concentrations in streams when extreme accuracy is not warranted.

Subject Headings: Dyes | Calibration | Curvature | Temperature effects | Water temperature | Field tests | Rivers and streams | Temperature distribution

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