American Society of Civil Engineers


Arsenite Oxidation by Batch Cultures of Thiomonas Arsenivorans Strain b6


by Aniruddha Dastidar, (Graduate student, Dept. of Civil Engineering, Univ. of Kentucky, 161 Raymond Building, Lexington, KY 40506.) and Yi-Tin Wang, (Professor, Dept. of Civil Engineering, Univ. of Kentucky, Lexington, KY 40506-0281.)

Journal of Environmental Engineering, Vol. 135, No. 8, August 2009, pp. 708-715, (doi:  http://dx.doi.org/10.1061/(ASCE)EE.1943-7870.0000020)

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Document type: Journal Paper
Abstract: Arsenite [As(III)] oxidation by Thiomonas arsenivorans Strain b6 was investigated in batch reactors at pH 6 and 30°C over As(III) concentrations ranging from 10 to 1,000 mg/L in the absence of added organic carbon. Strain b6 completely oxidized As(III) to arsenate [As(V)] during exponential growth phase for lower levels of As(III) concentrations (≤100 mg/L). At higher levels of 500 and 1,000 mg/L, As(III) oxidation was observed mostly in the exponential phase but continued into the stationary phase of growth. The Haldane substrate inhibition model was used to estimate biokinetic parameters for As(III) oxidation. The best fit parameters of half saturation constant Ks=33.2±1.87 mg/L, maximum specific substrate utilization rate k=(0.85±0.18)-mg As(III)/mg dry cell weight/h, substrate inhibition coefficient Ki=602.4±33.6 mg/L, yield coefficient Y=(0.088±0.0048)-mg cell dry weight/mg As(III), and endogenous decay coefficient kd=0.006±0.002 h–1 were obtained using the Adams-Bashforth-Moulton algorithm and nonlinear regression technique. Sensitivity analysis revealed that Y and Ki are the most sensitive to model predictions, while kd is the least sensitive to model simulation at both low and high concentrations of As(III).


ASCE Subject Headings:
Oxidation
Arsenic
Parameters
Toxicity