American Society of Civil Engineers


Impact of Initial pH and Linoleic Acid (C18:2) on Hydrogen Production by a Mesophilic Anaerobic Mixed Culture


by Srimanta Ray, (Grad. Student, Dept. of Civ. and Envir. Engrg., Univ. of Windsor, 335 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: rays@webmail1.uwindsor.ca), Nabin Chowdhury, (Grad. Student, Dept. of Civ. and Envir. Engrg., Univ. of Western Ontario, London, Ontario, Canada N6A 5B9. E-mail: nchowdh3@uwo.ca), Jerald A. Lalman, (corresponding author), (Assoc. Prof., Dept. of Civ. and Envir. Engrg., Univ. of Windsor, 334 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4 E-mail: lalman@uwindsor.ca), Rajesh Seth, (Asst. Prof., Dept. of Civ. and Envir. Engrg., Univ. of Windsor, B0& Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: rseth@uwindsor.ca), and Nihar Biswas, (Prof., Dept. of Civ. and Envir. Engrg., Univ. of Windsor, 147 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: biswas@uwindsor.ca)

Journal of Environmental Engineering, Vol. 134, No. 2, February 2008, pp. 110-117, (doi:  http://dx.doi.org/10.1061/(ASCE)0733-9372(2008)134:2(110))

     Access full text
     Purchase Subscription
     Permissions for Reuse  

Document type: Journal Paper
Abstract: Hydrogen production from glucose using a mixed anaerobic culture was assessed under batch conditions by adjusting the initial pH and adding linoleic acid (LA). At an initial pH of 5.0, hydrogen (1.9 mol H2 mol-1 glucose) was detected after the first glucose injection in the controls (no LA added). At the latter initial pH and in cultures fed 2,000 mg l-1 LA, the yield reached a maximum of 2.4 mol H2 mol-1 glucose when glucose was injected again. Hydrogen was detected after the second glucose injection in cultures with the initial pH adjusted to 5.0 and 6.0. In cultures receiving LA and adjusted to pH 7.6, the hydrogen yield reached 1.4 mol H2 mol-1 glucose when glucose was injected again. Acetate, propionate, and butyrate were detected under all conditions; however, the quantities were variable and dependent on the conditions examined. In comparison to the amount of volatile fatty acids produced, relatively low quantities of alcohols (ethanol, i-propanol, n-butanol, and i-butanol) were detected during the initial lag phase of 96 to 120 h.


ASCE Subject Headings:
Anaerobic treatment
Hydrogen
Gas
pH
Acids
Waste management