American Society of Civil Engineers


Hydraulic Conductivity of Compacted Soil Treated with Biofilm


by Michael L. Dennis, A.M.ASCE, (Engr., Shephard-Wesnitzer, Flagstaff, AZ 86004) and John P. Turner, M.ASCE, (Prof., Dept. of Civ. and Arch. Engrg., Univ. of Wyoming, P.O. Box 3295, Laramie, WY 82071)

Journal of Geotechnical and Geoenvironmental Engineering
, Vol. 124, No. 2, February 1998, pp. 120-127, (doi:  http://dx.doi.org/10.1061/(ASCE)1090-0241(1998)124:2(120))

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Document type: Journal Paper
Abstract: A laboratory study was conducted to evaluate the feasibility of creating low-permeability waste containment barriers using soil treated with bacteria to produce a plugging biofilm. The effects of exopolysaccharide-producing bacteria on the hydraulic conductivity (k) of a compacted silty sand were determined. Values of k for this soil without bacterial treatment ranged from 10–5 to 10–6 cm/s, depending on molding moisture content. Soil specimens were molded with a bacterial and nutrient solution, compacted at optimum moisture content, permeated with nutrient solutions, and tested for k using a flexible-wall permeameter. Significant reductions in k were observed, and most specimens reached a stable final k of 10–8 cm/s. The durability of the biofilm was tested by permeation with saline, acidic, and basic solutions, and by subjecting specimens to wet-dry conditions. In most cases these chemical and physical challenges had little or no effect on the reduced k. Results of these tests demonstrate that biofilm treatment may be a feasible technology for creating waste containment barriers in soil.


ASCE Subject Headings:
Bacteria
Biofilm
Biological processes
Compacted soils
Hydraulic conductivity
Liners
Waste storage