Biodegradation of 2,4-Dinitrotoluene in a Two Stage Systemby S. L. VanderLoop, Univ of Cincinnati, Cincinnati, United States,
M. T. Suidan, Univ of Cincinnati, Cincinnati, United States,
M. A. Moteleb, Univ of Cincinnati, Cincinnati, United States,
S. W. Maloney, Univ of Cincinnati, Cincinnati, United States,
Abstract: An anaerobic/anoxic fluidized-bed GAC bioreactor in series with an activated sludge reactor was used to treat 2,4-Dinitrotoluene (2,4-DNT). A simulated high strength wastewater solution of 2,4-DNT, ethanol, and ethyl ether as well as carbonate buffer and nutrient solutions were fed to the anaerobic/anoxic reactor. The environment in the fluidized-bed reactor was varied to determine its effect on 2,4-DNT biodegradation. The effluent from this reactor was treated further in an activated sludge system. Methanogenic operation of the fluidized-bed resulted in stoichiometric transformation of 2,4-DNT to 2,4-diaminotoluene (2,4-DAT). The 2,4-DAT was completely mineralized by the activated sludge. The system failed to transform the 2,4-DNT under anaerobic conditions without addition of a primary substrate. The effects of operating the first stage under nitrate reducing conditions with a primary substrate is currently being investigated.
Subject Headings: Fluidized bed technology | Activated sludge | Substrates | Nitrates | Anaerobic processes | Bioreactors
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